Assessing passive transfer immunity in newborn calves | Dellait

Álvaro García

Calves are born without antibodies. Their survival depends on how quickly and effectively they receive colostrum, their only source of immune protection. Every hour after birth, antibody absorption drops by about 3–4%, and after 24 hours, the gut can no longer absorb immunoglobulins (IgG). Feeding 3–4 liters of high-quality colostrum within two hours is the most profitable and life-saving act on a dairy farm.

Quality colostrum: Immunity you can measure

Use a Brix refractometer to check quality.

  • ≥ 22% Brix = high IgG concentration
  • 18–21% = moderate
  • < 18% = low; not suitable as first feeding

Identify cows producing the best colostrum.

The cost of failure

When calves fail to acquire passive immunity (FPT), their risk of disease and death multiplies. Economic losses include:

  • $50- $100 extra rearing cost per calf
  • $80 + per head mortality loss
  • Delayed growth and later breeding age

By contrast, a Brix tester (~$200) pays for itself within weeks. When combined with proper feeding, the ROI exceeds 10:1.

Clean colostrum = Better absorption

Dirty equipment ruins good colostrum. Bacteria interfere with IgG absorption and can infect calves directly. Follow these steps:

  • Wash and sanitize all bottles and nipples.
  • Avoid pooling milk from multiple cows.
  • Cool to ≤ 4°C within one hour.
  • Heat-treat at 60°C for 60 minute, this kills pathogens without damaging antibodies.

A clean start means fewer sick calves and reduced antibiotic use.

Simple habits that save lives

Mortality can be cut in half by consistent, low-cost hygiene:

  • Navel dipping reduces deaths from 7% to 3%.
  • Dry bedding and good ventilation prevent pneumonia and scours.
  • Disinfecting pens between calves lowered deaths from 5.8% to 2.6% in research herds.
  • Consistency, not complexity, drives success.

When technology helps

Automatic calf feeders equipped with drinking-speed sensors can detect illness 24 hours before visible symptoms. Farms using these alerts reduced mortality from 6.6% to 2.4% (Jorgensen et al., 2017).

Maternal and environmental influences

Multiparous cows (second lactation or older) often produce richer, higher-IgG colostrum. Proper dry cow nutrition, including vitamins A and E, and timely vaccination, improve both colostrum quality and calf immunity. Cold, humid barns, double treatment rates, simple investments in drainage, bedding, and air movement pay big health dividends.

The calf program’s scorecard

Key performance indicators (KPIs) for colostrum management and calf health:

  • Calves fed colostrum ≤ 2 h after birth  ≥ 90%
  • Colostrum ≥ 22% Brix  ≥ 80%
  • Calves ≥ 8.4% serum Brix (STP) ≥ 80%
  • Pre-weaning mortality < 3%

Review these numbers monthly. Small improvements save thousands of dollars per year.

Growing calves the right way

Healthier calves grow faster, reach puberty sooner, and produce more milk later. Once they eat ≥ 1.5–2.0 kg of starter for two consecutive days, they are ready to wean. Pair housing, good starter feed, and smooth weaning transitions are critical for lifetime performance.

Collecting serum from calf blood (no centrifuge) 

Evaluating serum total protein with a refractometer is a reliable, low-cost way to confirm whether a calf received adequate colostrum. Even without a centrifuge, clean serum can be collected easily on-farm using plain tubes.

  • Draw 2–5 mL of venous blood into a plain red-top tube (no additives).
  • Avoid hemolysis, use gentle suction, and do not shake the sample.
  • Label with calf ID and collection time.
Why red-top tubes?

Red-top vials contain no anticoagulants, allowing blood to clot naturally so that clear serum separates from the clot. This serum represents the true fluid portion needed for accurate refractometer readings of total protein or passive immunity.
Other commonly colored tubes contain additives that interfere with testing:

  • Green-top (heparin): prevents clotting by activating antithrombin, leaving plasma instead of serum and altering protein readings.
  • Purple/lavender-top (EDTA): binds calcium to stop coagulation but also distorts refractometer readings due to chelating effects.
  • Blue-top (sodium citrate): dilutes the sample with buffer solution used for coagulation studies, producing falsely low protein values.

Only red-top tubes ensure a clean serum layer suitable for passive transfer testing.

Let it clot
  • Keep tubes upright at room temperature for 30–60 minutes until a firm clot forms.
  • Let stand another 30–60 minutes for the clear serum to separate fully.
  • Use a pipette or small syringe to remove the clear upper layer only.
  • Avoid red blood cells or cloudy/pink serum.
Ready for the refractometer
  • One drop of clear serum is enough for testing.
  • If the serum is cloudy or pink (hemolyzed), discard and recollect a fresh sample.
Tips:
  • Allow 1-2 hours total clotting time.
  • Keep tubes clean and upright.
  • Record the calf ID and result immediately after reading.

Testing serum total protein ensures your colostrum and feeding protocols are truly effective. Calves with ≥ 5.5 g/dL (or ≥ 8.4 % Brix) are considered to have successful passive transfer, while lower results indicate the need to review colostrum quality, timing, and hygiene.

Final message

Calf health is not luck, it is management. Mortality drops when farmers focus on timing, cleanliness, and measurable quality. Colostrum is the calf’s first vaccine and the herd’s first profit margin. The care given in the first two hours defines the next two years.

The full list of references used in this article is available upon request.

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